mouse anti psd 95  (NeuroMab)

Journal: bioRxiv

Article Title: Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease

doi: 10.64898/2025.12.06.692741

Figure Lengend Snippet: a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

Article Snippet: Primary antibodies and working dilutions used were as follows: rabbit polyclonal anti-Synapsin I antibody (Millipore Sigma, AB1543, 1:1000); mouse monoclonal anti-PSD95 antibody (Novus Biologicals, NB300-556, 1:1000); mouse monoclonal anti-STAT3 antibody (Cell Signaling, 9139, 1:1,000); rabbit monoclonal phospho-STAT3 (Tyr705) antibody (Cell Signaling, Cat# 9145, 1:1,000); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (Millipore Sigma, G3893, 1:2,000); rabbit polyclonal anti-Iba1 antibody (FUJIFILM Wako Pure Chemical Corporation, 019-19741, 1:1,000); rabbit polyclonal anti-complement C3 antibody (Invitrogen, PA5-21349, 1:1,000); rat monoclonal anti-CD16/CD32 antibody (Invitrogen, 14-0161-82, 1:1,000); mouse monoclonal anti-β-Amyloid antibody (6E10) (BioLegend, 803001, 1:1,000).

Techniques: Western Blot, Two Tailed Test, Immunofluorescence

Journal: bioRxiv

Article Title: Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease

doi: 10.64898/2025.12.06.692741

Figure Lengend Snippet: a , Percentage of time in the novel arm, total distance traveled, and arm entries in the first Y-maze test. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 9; CamK-PARIS (+Napabucasin), n = 10). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. b , Mean speed during Barnes maze training. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 8; CamK-PARIS (+Napabucasin), n = 8). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. c , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of mice. Scale bar, 50 µm. d , e , Quantification of SYNAPSIN I–positive area and PSD95–positive area from c . Data are mean ± SEM (Control, n = 33; CamK-PARIS, n = 32; CamK-PARIS (+Napabucasin), n = 31; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph. f , Representative co-immunostaining images of GFAP (red), C3 (green), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. g–i , Quantification of GFAP and relative C3 fluorescence from f . Data are mean ± SEM (Control, n = 38; CamK-PARIS, n = 47; CamK-PARIS (+Napabucasin), n = 51; images from 7–12 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. j , Representative co-immunostaining images of IBA1 (green), CD16/CD32 (red), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. k , l , Quantification of IBA1 and relative CD16/CD32 fluorescence from j . Data are mean ± SEM (Control, n = 43; CamK-PARIS, n = 45; CamK-PARIS (+Napabucasin), n = 50; images from 7–10 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

Article Snippet: Primary antibodies and working dilutions used were as follows: rabbit polyclonal anti-Synapsin I antibody (Millipore Sigma, AB1543, 1:1000); mouse monoclonal anti-PSD95 antibody (Novus Biologicals, NB300-556, 1:1000); mouse monoclonal anti-STAT3 antibody (Cell Signaling, 9139, 1:1,000); rabbit monoclonal phospho-STAT3 (Tyr705) antibody (Cell Signaling, Cat# 9145, 1:1,000); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (Millipore Sigma, G3893, 1:2,000); rabbit polyclonal anti-Iba1 antibody (FUJIFILM Wako Pure Chemical Corporation, 019-19741, 1:1,000); rabbit polyclonal anti-complement C3 antibody (Invitrogen, PA5-21349, 1:1,000); rat monoclonal anti-CD16/CD32 antibody (Invitrogen, 14-0161-82, 1:1,000); mouse monoclonal anti-β-Amyloid antibody (6E10) (BioLegend, 803001, 1:1,000).

Techniques: Control, Immunofluorescence, Immunostaining, Fluorescence